PROPAGATION AND INFECTIVITY TITRATION OF THE GIFU-1 STRAIN OF CHICKEN ANEMIA AGENT IN A CELL-LINE (MDCC-MSB1) DERIVED FROM MAREKS-DISEASE LYMPHOMA

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 23  (1) , 13-20
Abstract
Chicken anemia agent (CAA) propagated in an established cell line derived from Marek''s disease (MD) lymphoma (MDCC-MSB1). When passaged 19 times in MDCC-MSB1 cell cultures, it produced anemia of the same severity in chicks as it did before passage. Titration of the infectivity of CAA was performed successfully with subcultures of MDCC-MSB1 cell cultures which had been inoculated with serial 10-fold dilutions of infected material. No infected cultures could be subcultured. The propagation of CAA was also demonstrated in the MD cell line, MDCC-JP2, and the avian lymphoid leukosis (LL) cell line, LSCC-1104B1, but not in the 2 MD cell lines, MDCC-RP1 and MDCC-BP1, or in the 2 LL cell lines, LSCC-1104X5 and LSCC-TLT. No CAA propagated in cell cultures prepared from skin and muscle, liver or brain of chick embryos, or kidney, thymus, bursa of Fabricius, bone marrow or white blood cells of chickens.