Extraction and Separation of Neutral Milk Lipids by Thin-Layer Chromatography

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Abstract
A technique has been developed whereby milk is spotted directly on a one-millimeter Silica Gel G plate, and the neutral lipid group is separated into its respective classes with an appropriate developing solvent. The acceptability of the technique was determined by comparing thin-layer patterns of extracted milk lipids with milk spotted directly on a plate. It was apparent from the thin-layer chromato-grams that identical lipid patterns were obtained regardless of whether the sample was spotted as an extract or whole milk. It seems probable that this technique may have merit as a simple, rapid means of studying biological reactions in such aqueous systems as blood and urine.