Construction of an infectious DNA clone of the Y 1 strain of canine parvovirus and characterization of the virus derived from the clone

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Abstract
We have cloned genome fragments of canine parvovirus strain Y 1 from replicative-form DNA and double-stranded DNA synthesized from virion DNA in vitro, and constructed a recombinant plasmid containing a full-length Y 1 genome (pCPVY 1). When this recombinant plasmid was transfected into cell cultures, an infectious virus could be recovered. To characterize this pCPVY 1-derived virus, its biological properties were compared with those of the parental strain. No difference was observed between them in antigen expression, viral DNA replication, hemagglutination ability, and virus multiplication, indicating that the virus derived from the infectious plasmid inherited the biological properties of the authentic Y 1 strain. Therefore, this recombinant plasmid appears to be useful for reverse genetics of canine parvovirus.