Biosynthesis of Dehydroisoandrosterone Sulfate by Human Adrenocortical Tissue
- 1 January 1963
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 23 (1) , 90-94
- https://doi.org/10.1210/jcem-23-1-90
Abstract
The conversion of 7α-H3-dehydroisoandrosterone to its sulfate ester by homogenates of an adrenocortical carcinoma is described. 7α-H3-dehydroisoandrosterone sulfate was characterized by its chromatographic behavior in 2 partition systems and its reaction with the methylene blue reagent for sulfates. The conjugate was recrystallized to constant specific activity and its identity was further established by its infrared spectrum. The steroid moiety was obtained by solvolysis and was identified as dehydroisoandrosterone by infrared analysis and recrystallization to constant specific activity.Keywords
This publication has 7 references indexed in Scilit:
- Precursors of the Urinary ll-Desoxy-17-ketosteroids: Estimation of the Secretory Rate of DehydroisoandrosteroneJournal of Clinical Endocrinology & Metabolism, 1962
- Studies of Conjugated 17-Ketosteroids in a Case of Adrenal TumorJournal of Clinical Endocrinology & Metabolism, 1962
- Isolation and Characterization of Aldosterone Metabolites from Human Urine; Two Metabolites Bearing a Bicyclic Acetal Structure*Biochemistry, 1962
- The Conversion in Vivo of Dehydroisoandrosterone Sulfate to Androsterone and Etiocholanolone GlucuronidatesJournal of Biological Chemistry, 1961
- Separation of Steroid SulfokinasesJournal of Biological Chemistry, 1958
- Hydrolysis of Ketosteroid Hydrogen Sulfates by Solvolysis ProceduresJournal of Biological Chemistry, 1958
- The enzymic synthesis of steroid sulphatesBiochemical Journal, 1956