Monoclonal Antibodies Against Rat Immunoglobulin Kappa Chains

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Abstract
Monoclonal antibodies directed against rat kappa light chains have been generated by immunizing SJL/J mice with soluble rat immunoglobulin, followed by fusion of immune spleen cells with the P3-X63-Ag8 myeloma cell line. Monoclonal antibodies from three of these hybridoma cell lines, MAR 18.5, 80.2, and 103.6, have been extensively characterized. MAR 18.5, 80.2, and 103.6 antibodies are of the λ2a x isotype, and bind strongly to protein A, allowing easy purification. Monoclonal antibody from clone 18.5 binds equally well to Ig of both Rl-la and Rl-lb allotypes, whereas 80.2 and 103.6 antibodies selectively bind to Rl-lb. These monoclonal antibodies can be FITC conjugated for use as a second antibody in indirect immunofluorescence assays, or radiolabeled for use in radio immunoassays requiring a specific antirat x antibody. The antiallotype specific monoclonal antibodies also may be of use in the study of rat immunoglobulin genetics.