Human Z α1‐antitrypsin accumulates intracellularly and stimulates lysosomal activity when synthesised in the Xenopus oocyte
- 22 April 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 183 (2) , 304-308
- https://doi.org/10.1016/0014-5793(85)80798-5
Abstract
Microinjection of human liver mRNA from a patient homozygous for α1-antitrypsin deficiency (PiZZ) into Xenopus oocytes led to a 2–10-fold increase in lysosomal activity. Stimulation of lysosomal activity was not observed when mRNA from a normal human liver (α1-antitrypsin PiMM), or water was injected into the oocyte. This lysosomal activity was oocyte derived and was not due to translation products of the human liver mRNA. Thus a protein that accumulates intracellularly in the secretory pathway is capable of stimulating lysosomal activity.Keywords
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