STUDIES ON INVITRO COLONY FORMATION BY MOUSE BONE-MARROW CELLS USING DIFFERENT INFLAMMATORY PLEURAL EXUDATES - RELATION TO COLONY STIMULATING FACTOR (CSF)

  • 1 January 1980
    • journal article
    • research article
    • Vol. 8  (2) , 166-173
Abstract
An inflammatory exudate obtained in Swiss mice 3 h after intrapleural injection of dextran increased the number of cultivated peritoneal macrophages in S phase. This exudate also stimulated the formation of colonies from mice bone marrow progenitors of macrophages and granulocytes in methylcellulose culture. The stimulating activity of this acute inflammatory exudate was compared with that of colony-stimulating factor (GM.CSF). Qualitative and quantitative results showed that the biological activity of the mitogenic factor of this inflammatory exudate, inflammatory mitogenic factor (IMF) was very close to GM.CSF at optimal concentration, but when used at the same concentration, the exudate was less active than GM.CSF. A stimulating activity was also found with another inflammatory pleural exudate induced by calcium pyrophosphate. The comparative kinetics of the action of the 2 exudates on CFUc [granulocyte-macrophage colony forming unit] appeared alike but the rise was earlier with calcium pyrophosphate. A growth factor for monocytes-macrophages is apparently released in different acute inflammatory exudates.

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