Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli.
- 1 July 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (13) , 4336-4340
- https://doi.org/10.1073/pnas.82.13.4336
Abstract
The nucleotide sequence of a 2.8-kilobase fragment of the E. coli chromosome containing the umuDC genes was determined. The DNA sequence specifies 2 open reading frames of 417 and 1266 nucleotides encoding proteins with calculated MW of 15,063 and 47,677, respectively. From these and the previous results of genetic and biochemical studies on the cloned genes, the former is the umuD and the latter is the umuC gene. In vitro transcription of the regulatory region of the umu operon revealed that upstream of the coding region there is a promoter-operator complex having the consensus sequence, CTGTATATAAAAACAG, of an SOS box, transcription of the umu operon begins at an adenine residue in the SOS box, and the LexA protein binds to the operator region, with an equilibrium Kd of 0.2, thereby repressing the transcription of the umuDC genes. These results indicate that the expression of the umu operon is under the coordinated control of the recA lexA gene products.This publication has 36 references indexed in Scilit:
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