Analysis of the water-soluble products of phosphatidylcholine breakdown by ion-exchange chromatography. Bombesin and TPA (12-O-tetradecanoylphorbol 13-acetate) stimulate choline generation in Swiss 3T3 cells by a common mechanism
- 15 October 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 263 (2) , 581-587
- https://doi.org/10.1042/bj2630581
Abstract
A method for the rapid and quantitative separation of glycerophosphocholine, choline phosphate and choline upon ion-exchange columns is described. The method has been utilized to examine the stimulation of phosphatidylcholine breakdown in quiescent Swiss 3T3 cells in response to bombesin and 12-O-tetradecanoylphorbol 13-acetate (TPA). The stimulated generation of choline is shown to precede that of choline phosphate, with no effect upon glycerophosphocholine levels; but was attenuated in cells in which protein kinase C activity was down-regulated. The results thus suggest that stimulation of the cells with either bombesin or TPA activates phospholipase D-catalysed phosphatidylcholine breakdown by a common mechanism involving the activation of protein kinase C.This publication has 25 references indexed in Scilit:
- Formation of diacylglycerol by a phospholipase D-phosphatidate phosphatase pathway specific for phosphatidylcholine in endothelial cellsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1988
- The phosphatidylcholine pathway of diacylglycerol formation stimulated by phorbol diesters occurs via phospholipase D activationFEBS Letters, 1988
- Vasopressin is the only component of serum-free medium that stimulates phosphatidylcholine hydrolysis and accumulation of diacylglycerol in cultured REF52 cellsBiochemical and Biophysical Research Communications, 1988
- Activation of phospholipase D by chemotactic peptide in HL-60 granulocytesBiochemical and Biophysical Research Communications, 1988
- Inositol lipids and cell proliferationBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1987
- Bombesin and phorbol ester stimulate phosphatidylcholine hydrolysis by phospholipase C: Evidence for a role of protein kinase CJournal of Cellular Physiology, 1987
- Growth factor-like action of phosphatidic acidNature, 1986
- Reduction of epidermal growth factor receptor affinity by heterologous ligands: Evidence for a mechanism involving the breakdown of phosphoinositides and the activation of protein kinase CBiochemical and Biophysical Research Communications, 1984
- Disappearance of Ca2+-sensitive, phospholipid-dependent protein kinase activity in phorbol ester-treated 3T3 cellsBiochemical and Biophysical Research Communications, 1984
- Regulation of phosphatidylcholine biosynthesis by mitogenic growth factorsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984