An improved method for HBcAg demonstration in paraffin‐embedded liver tissue

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Abstract
Factors influencing the preservation of hepatitis B core antigen (HBcAg) in formalin-fixed, paraffin-embedded human liver tissue were analyzed. Fixation time, choice of the embedding medium, deparaffinization and selection of anti-hepatitis B core antigen antibody (anti-HBc) proved to be critical variables. For optimal results, fixation in 4% buffered formalin for no longer than 24-48 h, the use of wax with a low melting point, a heating step prior to deparaffinization and a search for anti-HBc specifically reactive for paraffinated HBcAg are recommended.