Direct demonstration of genetic alterations at the dihydrofolate reductase locus after gamma irradiation.
Open Access
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 2 (1) , 93-96
- https://doi.org/10.1128/mcb.2.1.93
Abstract
Gamma ray-induced mutants of Chinese hamster ovary cells lacking dihydrofolate reductase activity were screened for DNA sequence changes at the locus specifying this activity by using a cloned cDNA probe. Two of nine mutants screened displayed an altered restriction fragment pattern suggesting the occurrence of DNA deletions or rearrangements.This publication has 24 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Do radiation-induced thioguanine-resistant mutants of cultured mammalian cells arise by HGPRT gene mutation or X-chromosome rearrangement?Nature, 1978
- Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductaseNature, 1978
- A complete immunoglobulin gene is created by somatic recombinationCell, 1978
- The transfer and stable integration of the HSV thymidine kinase gene into mouse cellsCell, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Re-analysis of radiation-induced specific locus mutations in the mouseNature, 1976
- Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cellsSomatic Cell and Molecular Genetics, 1976
- Two different species of dihydrofolate reductase in mammalian cells differentially resistant to amethopterin and methasquin.1972
- Definition of functional units in a small chromosomal segment of the mouse and its use in interpreting the nature of radiation-induced mutationsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1971