Cytogenetic analysis of the interspecific hybrid Vigna radiata × V. umbellata

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Abstract
Vigna radiata, V. umbellata and their interspecific F1 were examined cytologically. Meiosis in the parents was mostly normal, but occasional multipolar divisions were observed at diakinesis and metaphase I with split spindles showing 5/6 segregations. Secondary associations at metaphase I showed six configurations per cell; five of these had two bivalents paired tightly side-by-side with the sixth configuration having a single bivalent. Meiosis in the interspecific hybrid was irregular. The mean pairing at metaphase I was 13.40 univalents, + 3.95 bivalents, + 0.18 trivalents, + 0.01 quadrivalents. Spindle abnormalities were observed in which the bivalents were segregated from the univalents. The number of microspores formed from each microsporocyte was irregular with 42 percent dyads, 9 percent triads, 39 percent tetrads, and 10 percent six-to-eight celled. Pollen stainability in the interspecific hybrid was low (1.5 percent); however, the size of the stainable pollen was noticeably larger than that of the parent species.