Oestrogen receptor-like binding in the surface germinal epithelium of the rat ovary
- 1 December 1982
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 95 (3) , 377-385
- https://doi.org/10.1677/joe.0.0950377
Abstract
The surface germinal epithelium of the rat ovary was isolated and grown in vitro. Cytosol from cultured cells contained a saturable component, of sedimentation coefficient 7·5–8·5S, which bound oestrogenic and antioestrogenic substances with high affinity (Kd 400 pmol/l). Nuclei isolated from cells exposed to [3H]oestradiol contained radioactivity partially susceptible to KC1 extraction. Sedimentation analysis of the KC1 extract showed that [3H]oestradiol was associated with a moiety of sedimentation coefficient 4·5–5S.This publication has 6 references indexed in Scilit:
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