DEVELOPMENT OF COLLAGEN IN THE FROG EMBRYO

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Abstract
Using the hydroxyproline content as a means of detecting collagen, the early history of this fibrous protein was traced in the frog embryo, Rana pipiens. Hydroxyproline 1st appears between Stages 21 and 22 (Shumway, W., Anat. Rec. 78: 139-147, 1940), the latter containing about 0.4 [mu]g per embryo, and increases linearly at least through Stage II (Taylor, A.C. and Kollros, J. J., Anat. Rec. 94: 7-24, 1946.), when about 5 [mu]g hydroxyproline per embryo are present. These hydroxyproline values correspond to 4 and 50 [mu]g of collagen, which comprises about 0.3 and 5%, respectively, of the fat-free dry tissue weights. Results suggests that collagen determination is particularly suitable for a study of embryonic chemodifferentation.