The 9-fluorenylmethyloxycarbonyl group as a 5?-OH protection in oligonucleotide synthesis
- 1 May 1989
- journal article
- research article
- Published by Wiley in Biopolymers
- Vol. 28 (5) , 965-973
- https://doi.org/10.1002/bip.360280505
Abstract
Oligo‐DNAs are synthesized on a solid support using the 9‐fluorenylmethyloxycarbonyl group as a 5′‐OH base labile protection. The synthesis of the pure protected nucleotides, a relevant phosphoramidite‐type strategy of coupling, and the optimization of the deprotection steps are described. This new synthetic method is an alternative to the standard protocol that avoids acidic conditions.This publication has 12 references indexed in Scilit:
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