CONJUGATED OESTROGENS. III

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Abstract
The urine was collected from postmenopausal women who had received estradiol-4-14C by intravenous injection. Hydrolysis was carried out for different periods of time and with various concentrations of enzyme ([beta]-glu-curonidase) and the relative distribution of several hydrolyzed estrogens determined under these conditions. The estrogens reported are 2-methoxyestrone, estrone, estradiol, ketols (16[alpha]-hydroxyestrone plus 16-ketoestradiol), epiestriol and estriol. The first order rate constant for estrone was particularly high in comparison with those of the other estrogens. Two plateaus were observed on the curves of the hydrolysis of several estrogens (ketols, epiestriol and estriol) when plotted as a function of time, possibly as a result of multiple conjugation of these estrogens.