Stimulation of β2-Adrenergic Responsiveness by Follicle-Stimulating Hormone in Rat Granulosa Cellsin Vitroandin Vivo*

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Abstract
The development of adrenergic responsiveness in ovarian granulosa cells cultured in the presence of androstenedione was investigated. Progesterone production by granulosa cells obtained from immature hypophysectomized diethylstilbestrol-treated rats was stimulated by FSH, but was only minimally affected by various adrenergic agents. In contrast, FSH treatment for 2 days in vivo or in vitro resulted in an increase in the adrenergic responsiveness of granulosa cells. Subsequent treatment with (−)epinephrine, (−)norepinephrine, or (−)isoproterenol (a potent β-adrenergic agonist) resulted in time- and dosedependent increases in progesterone production. The adrenergic agents enhanced the effects of hCG and PRL with respect to progesterone production, but were without effect on estrogen production or the maintenance of LH/hCG receptors in FSHprimed granulosa cells. A selective β2-adrenergic agonist (terbutaline) stimulated progesterone production in FSH-treated granulosa cells, whereas a β1-agonist (dobutamine) was 10,000-fold less effective. Furthermore, progesterone production induced by (−)epinephrine was blocked by a selective β2-antagonist (IPS 339), but the β1-antagonist (practolol) was 7,000-fold less effective. These results suggest that FSH treatment in vivo and in vitro increases β2-adrenergic responsiveness in ovarian granulosa cells and that this functional responsiveness is coupled to progesterone, but not to estrogen, biosynthesis.