THE METABOLISM OF CITRATE-C14 IN NORMAL AND IN FLUOROINHIBITOR-POISONED RATS*

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Abstract
An estimate of the rate of turnover and the metabolic fate of the blood citrate was accomplished with the aid of intravenously administered citrate-l,5-C14 Three hours after injection of the Radioactive compound, 55% of the C14 was recovered in the respiratory CO2 and 35% of the label was found in the urinary citrate. Approximately 1% of the label was incorporated into protein and lipid of the kidneys and liver. The incorporation of the radioactive carbon into the kidney fractions was greater than into the corresponding liver fractions. The effect of prior administration of each of the inhibitors, fluoroacetate and fluorocitrate, on the metabolism of the citrate-C14 was studied. Experiments with the intact animal and with liver and kidney slices indicated a difference in the mechanism of action of these metabolic inhibitors.