Cloning and Expression of a Cocaine-Sensitive Dopamine Transporter Complementary DNA

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Abstract
A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and γ-aminobutyric acid transporters. The expressed complementary DNA confers transport of [ 3 H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [ 3 H]CFT {[ 3 H]2β-carbomethoxy-3β-(4-fluorophenyl)tropane} to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.