Expression of the Escherichia coli Cell Division Gene sep Cloned in a λ Charon Phage
- 12 October 1979
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 206 (4415) , 220-222
- https://doi.org/10.1126/science.158216
Abstract
The Escherichia coli cell division gene sep, which probably codes for one of the penicillin-binding proteins, has been cloned into lambda Charon 10 to form a viable sep+ transducing phage. After infection with this hybrid phage, penicillin-binding protein 3 was overproduced and incorporated into the E. coli inner membrane.Keywords
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