Oxygen transfer rates in a mammalian cell culture bioreactor equipped with a cell‐lift impeller

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Abstract
Measurements of kLa were carried out in 1. 5‐ and 5‐L New Brunswick Scientific CelliGen® bioreactors. The measured kLa in water were identical for both vessel sizes operated in similar condition. The mass transfer rate increased with temperature, mixing speed, and aeration rate, with this last parameter being the most significant. Surface aeration alone gave kLa values of 0. 4 to 1. 6 h−1. A 25% decrease in kLa was observed above an aeration rate of 1. 6 vvm. This was caused by the particular foam breaker of the CelliGen bioreactor. Measurements of kLa using a mammalian cell culture medium supplemented with 5% fetal calf serum (FCS) have confirmed the negative effect of the foam breaker on kLa The measured value in this medium was 1. 2 h−1 for all aeration rates, more than 60% of which was attributed to surface aeration.

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