Comparison of partial amino acid sequences of two protamine 2 variants from stallion sperm Structural evidence that the variants are products of different genes
- 13 February 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 244 (1) , 199-202
- https://doi.org/10.1016/0014-5793(89)81192-5
Abstract
Protamine 1 and two protamine 2 variants were isolated from stallion sperm and separated by acetic acid-urea gel electrophoresis. After electroblotting onto polyvinyldifluoride filters, their amino-terminal amino acid sequences were determined by pulse-liquid peptide sequencing. The sequences of the two protamine 2 variants are homologous but slightly different in length and amino acid composition and indicate for the first time the existence of two different genes for this protamine species.Keywords
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