Stabilized Romanowsky Blood Stain

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Abstract
The degradation of thiazine dyes which normally occurs in methanolic solution, as in the case of Romanowsky [human] blood stains, can be prevented by making the solution acidic. In a certain range of acidity, the stain precipitates in the form of monothiazine eosinate, but by making the solution sufficiently acidic, eosin is protonated and the precipitate cannot form. These observations were used to develop a blood stain which is stable, even at elevated temperatures, for several months. For use the stain is neutralized by a specially formulated fixative solution.