DNA INTERACTION WITH HYDROGEN-PEROXIDE - A METHOD FOR DETERMINING PYRIMIDINE-BASES IN DNA

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  • 1 January 1983
    • journal article
    • letter
    • Vol. 9  (12) , 1696-1698
Abstract
Incomplete modification with H2O2 of DNA fragments labeled at one terminus is useful for localization of C and T residues along the polynucleotide chain. The fragments can be split at modified residues with piperidine after H2O2 treatment. The rate of the reaction of the single-stranded fragments is extremely fast, much higher than that of the double-stranded DNA. Splitting of the DNA takes place at C if pH 7.4 and at T at pH 9.6. The modification can be used to investigate DNA structure and function.

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