Studies on the generative nuclear division. I. Comparative analysis of somatic chromosomes in Allium fistulosum by means of C- and Q-banding patterns.

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Abstract
A comparative study of the chromosomes from root tip cells and from pollen tube generative cells in A. fistulosum carried out by Giemsa C[constitutive heterochromatin]-banding techniques and fluorochromes. Pollen tubes were cultured on the inside surface of a spitz-type glass centrifugation tube containing a medium with 0.02% colchicine. In root tip chromosomes, C-banding techniques revealed deeply stained satellites and distal banded ends corresponding to the reduced fluorescence bands. Centromeric bands, proximal ones in the gap on the shortest submetacentric chromosomes (SM4) and additional interstitial ones (chromosomes SM4 and M3) were found occasionally in the C-banding patterns. Inter-plant variation of C-banding patterns was observed. No organ specificity was recognized in the karyotype and the C- and Q[quinacrine]-banding patterns between the chromosomes from root-tip cells and pollen tube generative cells. Difficulties in the banding techniques for the pollen tube generative nucleus are discussed.