Identification of an IgE-binding Region in Soybean Acidic Glycinin G1
Open Access
- 1 March 1999
- journal article
- research article
- Published by Taylor & Francis in Food and Agricultural Immunology
- Vol. 11 (1) , 83-90
- https://doi.org/10.1080/09540109999942
Abstract
The acidic polypeptide chain of soybean glycinin G1 was identified as one of the several IgE-binding proteins by immuno-blotting with pooled sera from soy-allergic individuals. The region of IgE-binding within glycinin G1 was narrowed by in situ digestion with endoproteinase GluC followed by immuno-blotting of peptide products. This procedure resulted in a single IgE-binding fragment of approximately 15 kDa corresponding to residues 192 to 306 of the acidic chain of glycinin G1.Keywords
This publication has 17 references indexed in Scilit:
- Identification of the Glycosylation Site of a Major Soybean Allergen,Gly mBd 30KBioscience, Biotechnology, and Biochemistry, 1996
- Molecular structure and immunological properties of food allergensTrends in Food Science & Technology, 1993
- Identification of Soy Protein Allergens in Patients with Atopic Dermatitis and Positive Soy Challenges: Determination of Change in Allergenicity after Heating or Enzyme DigestionPublished by Springer Nature ,1991
- Studies of Food Allergens: Soybean and Egg ProteinsPublished by Springer Nature ,1991
- Quantitative Electrotransfer of Proteins From Polyacrylamide Gels onto PVDF MembranesPublished by Elsevier ,1990
- Identification of Soybean Allergens by Immunoblotting with Sera from Soy-Allergic AdultsInternational Archives of Allergy and Immunology, 1990
- Characterization of the glycinin gene family in soybean.Plant Cell, 1989
- Allergenicity of major component proteins of soybean determined by enzyme-linked immunosorbent assay (ELISA) and immunoblotting in children with atopic dermatitis and positive soy challengesJournal of Allergy and Clinical Immunology, 1988
- Kunitz Soybean Trypsin InhibitorNew England Journal of Medicine, 1980
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970