Protein Kinase Cα Negatively Regulates Systolic and Diastolic Function in Pathological Hypertrophy

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Abstract
The protein kinase C (PKC) family is implicated in cardiac hypertrophy, contractile failure, and β-adrenergic receptor (βAR) dysfunction. Herein, we describe the effects of gain- and loss-of-PKCα function using transgenic expression of conventional PKC isoform translocation modifiers. In contrast to previously studied PKC isoforms, activation of PKCα failed to induce cardiac hypertrophy, but instead caused βAR insensitivity and ventricular dysfunction. PKCα inhibition had opposite effects. Because PKCα is upregulated in human and experimental cardiac hypertrophy and failure, its effects were also assessed in the context of the Gαq overexpression model (in which PKCα is transcriptionally upregulated). Normalization (inhibition) of PKCα activity in Gαq hearts improved systolic and diastolic function, whereas further activation of PKCα caused a lethal restrictive cardiomyopathy with marked interstitial fibrosis. These results define pathological roles for PKCα as a negative regulator of ventricular systolic and diastolic function.