Optical rotatory dispersion of E. coli ribosomes and their constituents

Abstract
Investigations have been made on the optical rotatory dispersion properties of E. coli ribosomes and their constituent RNA and proteins. The results indicate that (1) no conformational changes are involved in the formation of a 70S particle from the 50S and 308 subunits, (2) the E. coli ribosomal proteins are similar to most globular proteins with little α‐helix content, and (3) the conformation of RNA and proteins inside the ribosome is very similar to that in the free state.