Localization of tRNA genes of Drosophila melanogaster by in situ hybridization

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Abstract
Six purified tRNAs labeled with 125I by chemical or enzymatic methods were hybridized to polytene chromosomes of Drosophila melanogaster. The main chromosomal regions of hybridization were: tRNA GlyGGA , 58A, 84C, and 90E; tRNA Leu2 , 44E, 66B5-8, and 79F; tRNA Ser2b , 86A, 88A9-12, and 94A6-8; tRNA Thr3 , 47F and 87B; tRNA Thr4 , 93A1-2; and tRNA Tyr1γ , 19F, 22F-23A, 41, 50C1-4 and 85A. At 50C the hybridization of tRNA Tyr1γ was polymorphic in the giant strains. When the hybridization of three valine isoacceptors studied previously was re-investigated, it was found that only one hybridization site, 90BC, was shared between tRNA Val3b and tRNA Val4 . tRNA Val3a did not have any sites in common with the other two.