The nontranscribed chicken calmodulin pseudogene cross-hybridizes with mRNA from the slow-muscle troponin C gene.
Open Access
- 1 April 1987
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 7 (4) , 1549-1553
- https://doi.org/10.1128/mcb.7.4.1549
Abstract
A chicken calmodulin pseudogene with no introns was previously shown to hybridize under stringent conditions with an mRNA species present in skeletal and cardiac muscles, yet it would not hybridize to calmodulin mRNA (J. P. Stein, R. P. Munjaal, L. Lagace', E. C. Lai, B. W. O'Malley, and A. R. Means, Proc. Natl. Acad. Sci. USA 80:6485-6489, 1983). Using the pseudogene as a probe, we isolated a full-length cDNA corresponding to this mRNA from a chicken breast muscle library and showed by sequence analysis that it encodes slow-muscle troponin C and not the pseudogene product. Hybridization between the calmodulin pseudogene and slow-muscle troponin C cDNA is due to a short region of high homology in those nucleotides that encode helices B and C of troponin C and calmodulin. Genomic Southern analysis showed the calmodulin pseudogene and the gene for slow-muscle troponin C to exist as distinct single copies.This publication has 22 references indexed in Scilit:
- The sequences of an expressed rat α-tubulin gene and a pseudogene with an inserted repetitive elementNature, 1982
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Diverse Mechanisms in the Generation of Human β-Tubulin PseudogenesScience, 1982
- Evidence that a human β-tubulin pseudogene is derived from its corresponding mRNANature, 1982
- Processed genes: a dispersed human immunoglobulin gene bearing evidence of RNA-type processingNature, 1982
- cDNA clone analysis of six co-regulated mRNAs encoding skeletal muscle contractile proteins.Proceedings of the National Academy of Sciences, 1982
- Regulation of muscle differentiation: isolation and purification of chick actin messenger ribonucleic acid and quantitation with complementary deoxyribonucleic acid probesBiochemistry, 1980
- Synthesis of adult myosin light chains by embryonic muscle cultures.Proceedings of the National Academy of Sciences, 1980
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- The amino acid sequence of troponin C from chicken skeletal muscleFEBS Letters, 1976