Effect of Sodium Fluoride on Protein and DNA Synthesis, Ornithine Decarboxylase Activity, and Polyamine Content in LS Cells

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Abstract
Sodium fluoride exhibited a dose dependent inhibitory effect on protein and DNA synthesis at concentrations from 1.3 mM in growing LS cells. The activity of ornithine decarboxylase (ODC) was slightly stimulated by 0.5 mM-NaF, but inhibited at 1.3 mM and above. The reduced enzyme activity seemed to be due to a reduced de novo formation of the enzyme caused by an inhibition of the protein synthesis. In spite of a reduction in ODC-activity, fluoride had no effect on the cellular polyamine content during the experimental period (10 hours).