CONJUGATED OESTROGENS. II
- 1 July 1963
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 43 (3) , 361-374
- https://doi.org/10.1530/acta.0.0430361
Abstract
Celite 545, a diatomaceous earth, is coated with a high molecular weight secondary amine (Amberlite LA-1, Rohm and Haas, Phila., Pa.) and is used as a chromatographic support for the separation of various free and conjugated estrogens in urine and in extracts of urine. Elution is carried out with extremely dilute acids. Optimum separation was obtained with a column of Celite coated with LA-1 (formate) in which the conjugated estrogens in a concentrated extract of urine were eluted with a linear gradient (in the mixing chamber) of formic acid (pH 3.10 to 5.04).This publication has 5 references indexed in Scilit:
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