QUANTITATIVE PAPER CHROMATOGRAPHY OF CONJUGATED 17-KETOSTEROIDS IN PLASMA*

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Abstract
A method for the direct estimation of dehydroisoandrosterone sulfate and androsterone sulfate in plasma is presented. The procedure includes extraction, column and paper chromatography and direct quantification on paper by planimetric evaluation. It has been shown that the criteria for recovery, reproducibility and specificity of this method have been fulfilled. Values found for normal males in the reproductive age vary from 155 to 298 gamma/100 ml, and 29 to 92 gamma/100 ml for dehydroisoandrosterone sulfate and androsterone sulfate respectively. For females, the value for dehydroisoandrosterone sulfate varied from 85 to 166 gamma/100 ml, and for androsterone sulfate from< 15 to 45 gamma/100 ml. A limited number of samples obtained from female patients with virilizing disorders showed a significant elevation for one or both moieties.