Chromosomal Map of Pseudomonas putida PPN, and a Comparison of Gene Order with the Pseudomonas aeruginosa PAO Chromosomal Map

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Abstract
The generalized transducing phage Pf16h2 was used to confirm linkage relationships of chromosomal markers of P. putida previously determined from their time-of-entry in Hfr (high frequency of recombination) crosses, and to map new auxotrophic mutations. By means of spot matings using Hfr donors of known origin of transfer, catabolic markers forming part of a closely linked group of operons referred to as a superoperonic cluster were shown to be chromosomally located and their map positions determined. R-prime-mediated interspecific complementation was used to equate functionally 21 auxotrophic loci in P. putida and P. aeruginosa, and the distribution of these loci on the 2 genetic maps was compared. While both maps reveal that auxotrophic markers are largely restricted to .apprx. 40% of the chromosome and that auxotrophic markers of similar phenotype are not clustered, there is evidence of at least 7 chromosomal rearrangements since divergence from a presumed common ancestor.