STAINING BACTERIAL SMEARS WITH FLUORESCENT ANTIBODY IV

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Abstract
A specific fluorescein-labeled antibody reagent was prepared which stained all group A streptococcal strains tested and strains of no other streptococcal group. The reagent was prepared by adsorbing group A fluorescent antibody with group C organisms. The adsorption removed the cross-staining reaction with group C organisms but did not affect its affinity for group A cells. The reaction was performed on dried smears prepared from cultures and directly from throat swabs obtained from patients with acute streptococcal throat infections. More limited studies showed that specific fluorescent antibody reagents also could be prepared for streptococcal groups B, C, D, F, and G. The advantages of rapidity and economy offered by the use of specific streptococcal fluorescent antibody for grouping streptocci are discussed.

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