Reduction of acetylene and nitrogen gas by breis and cell-free extracts of soybean root nodules.

Abstract
By use of insoluble polyvinyl pyrollidone and a buffer containing ascorbate, breis have been prepared from soybean root nodules that catalyze the reduction of acetylene to ethylene. Cell-free extracts of nodule bacteriods catalyzed the reduction of acetylene to ethylene and of N2 to NH3. The reduction of both acetylene and N2 by cell-free extracts is dependent upon Na2S2O4 and an ATP generating system.

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