NITROGEN FIXATION: HYDROSULFITE AS ELECTRON DONOR WITH CELL-FREE PREPARATIONS OF AZOTOBACTER VINELANDII AND RHODOSPIRILLUM RUBRUM
- 1 March 1965
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 53 (3) , 532-539
- https://doi.org/10.1073/pnas.53.3.532
Abstract
Hydrosulfite as the electron donor for N2 reduction by cell-free preparations of A. vinelandii was demonstrated and optimal conditions for the reaction determined. Ammonia was the principal product of N2 reduction by the cell-free system. An adenosine triphosphate (ATP)-dependent hydrogenase in the preparations and its inhibition by N2 was demonstrated. Cell-free fixation with extracts of R. rubrum was demonstrated using hydrosulfite as the electron donor and an ATP-generating system.Keywords
This publication has 15 references indexed in Scilit:
- Nitrogen fixation: Cell-free system with extracts of azotobacterBiochemical and Biophysical Research Communications, 1964
- STABILITY OF NITROGEN-FIXING ENZYMES AND THE REACTIVATION OF A COLD LABILE ENZYMEProceedings of the National Academy of Sciences, 1963
- Photometabolism of Rhodospirillum rubrum: Light-dependent dissimilation of organic compounds to carbon dioxide and molecular hydrogen by an anaerobic citric acid cycleArchives of Biochemistry and Biophysics, 1962
- Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolismArchives of Biochemistry and Biophysics, 1961
- NITROGEN FIXATION BY CELL-FREE PREPARATIONS FROM MICROORGANISMSProceedings of the National Academy of Sciences, 1960
- A COMPARISON OF THE HYDROGENASE ACTIVITIES OF DIFFERENT MICROÖRGANISMSProceedings of the National Academy of Sciences, 1956
- A NEW PROCEDURE FOR ASSAY OF BACTERIAL HYDROGENASESJournal of Bacteriology, 1956
- DIRECT DEMONSTRATION OF AMMONIA AS AN INTERMEDIATE IN NITROGEN FIXATION BY AZOTOBACTERJournal of Biological Chemistry, 1953
- DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTIONJournal of Biological Chemistry, 1949