5-Nitroindole as an universal base analogue
Open Access
- 11 October 1994
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 22 (20) , 4039-4043
- https://doi.org/10.1093/nar/22.20.4039
Abstract
4-, 5- and 6-Nitroindole have been investigated and compared with 3-nitropyrrole as universal bases in oligodeoxynucleotides. Of these the 5-nitroindole derivative was found to be superior giving higher duplex stability, and behaving indiscriminately towards each of the four natural bases in duplex melting experiments. 3-Nitropyrrole, whilst not discriminating between the natural bases, was found to lead to considerable destabilisation of the duplexes, particularly when multiple substitutions were made, in contrast to the 5-nitroindole nucleoside.Keywords
This publication has 15 references indexed in Scilit:
- A universal nucleoside for use at ambiguous sites in DNA primersNature, 1994
- The synthesis and properties of oligodeoxyribonucleotides containing N6-methoxyadenineNucleic Acids Research, 1992
- Synthesis and duplex stability of oligonucleotides containing cytosine-thymine analoguesNucleic Acids Research, 1989
- 5-Fluorodeoxyuridine as an alternative to the synthesis of mixed hybridization probes for the detection of specific gene sequences.Proceedings of the National Academy of Sciences, 1988
- Studies on nucleic acid interactions I. Stabilities of mini-duplexes (dG2A4XA4G2.dC2T4YT4C2) and self-complementary d(GGGAAXYTTCCC) containing deoxyinosine and other mismatched basesNucleic Acids Research, 1986
- Synthesis and properties of oligonucleotides containing 2′-deoxynebularine and 2′-deoxyxanthosineNucleic Acids Research, 1986
- An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions.Journal of Biological Chemistry, 1985
- Base pairing involving deoxyinosine: implications for probe designNucleic Acids Research, 1985
- Synthesis and hybridization of dodecadeoxyribonucleotides containing a fluorescent pyridopyrimidine deoxynucleosideNucleic Acids Research, 1985
- Free energy of imperfect nucleic acid helicesJournal of Molecular Biology, 1973