ENDOGENOUS ANGIOTENSIN-I CONCENTRATION IN HUMAN-PLASMA

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 92  (3) , 353-362
Abstract
A reliable radioimmunoassay of endogenous plasma angiotensin I [AI] concentration, extracted on a Dowex-resin at pH 7.4, is described. The AI concentration in the pH 7.4-extracted plasma samples is significantly correlated with the AI concentration in the samples extracted at pH 5.5, the pH optimum of the in vitro reaction of human renin with human renin substrate. The presence of immunoreactive AI-like material in plasma and the relatively high cross-reaction of renin substrate with the anti-AI antiserum necessitates a chromatographic purification on a Dowex resin. The normal range found for AI in venous blood of apparently normal, male subjects was 25-143 pg/ml. A significant correlation (correlation coefficient = 0.78; P < 0.001) between P[plasma]A-I and PRA [plasma renin activity] was found in normal subjects. No differences (P > 0.10) between arterial and venous AI concentration were observed in hypertensive patients.

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