Immunospecificity of Non-histone Chromosomal Proteins in 89Sr-induced Osteogenic Sarcoma (Mouse)
- 1 May 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 81 (5) , 1549-1555
- https://doi.org/10.1093/oxfordjournals.jbchem.a131610
Abstract
Antibodies against non-histone chromosomal proteins from 89Sr-induced osteogenic sarcoma (mouse) were prepared by immunization of rabbits. The immunoreactivity of this antigen was then compared with those of non-histone chromosomal proteins from Ehrlich ascites tumor, normal mouse liver, and calf thymus by the method of quantitative microcomplement fixation. The non-histone chromosomal proteins of 98Sr-induced osteogenic sarcoma, fractionated by hydroxylapatite chromatography, exhibited significant affinity for the antibodies. Similar proteins from Ehrlich ascites tumor, normal mouse liver, or calf thymus were virtually inactive, indicating the tissue-specificity of 89Sr-induced osteogenic sarcoma proteins.This publication has 7 references indexed in Scilit:
- Association of neutral deoxyribonuclease with chromatin isolated from mammalian cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- The Isolation, Properties, and Possible Functions of Chromatin Acidic ProteinsJournal of Biological Chemistry, 1967
- MOLECULAR SIZE AND CIRCULARITY OF DNA IN CELLS OF MAMMALS AND HIGHER PLANTSProceedings of the National Academy of Sciences, 1965
- Quantitative Micro-Complement Fixation and Its Use in the Study of Antigenic Structure by Specific Antigen-Antibody InhibitionThe Journal of Immunology, 1961
- ENZYMATIC INCORPORATION OF RIBONUCLEOSIDE TRIPHOSPHATES INTO THE INTERPOLYNUCLEOTIDE LINKAGES OF RIBONUCLEIC ACIDProceedings of the National Academy of Sciences, 1960
- Isolation of pure and unaltered liver nuclei morphology and biochemical compositionExperimental Cell Research, 1956
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951