An elaboration on the syn–anti proton donor concept of glycoside hydrolases: electrostatic stabilisation of the transition state as a general strategy
- 21 December 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (2) , 302-312
- https://doi.org/10.1016/j.febslet.2004.12.021
Abstract
An in silico survey of all known 3D-structures of glycoside hydrolases that contain a ligand in the −1 subsite is presented. A recurrent crucial positioning of active site residues indicates a common general strategy for electrostatic stabilisation directed to the carbohydrate’s ring-oxygen at the transition state. This is substantially different depending on whether the enzyme’s proton donor is syn or anti positioned versus the substrate. A comprehensive list of enzymes belonging to 42 different families is given and selected examples are described. An implication for an early evolution scenario of glycoside hydrolases is discussedKeywords
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