STRANDEDNESS OF VICIA FABA CHROMOSOMES AS REVEALED BY ENZYME DIGESTION STUDIES
Open Access
- 1 July 1965
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 26 (1) , 125-135
- https://doi.org/10.1083/jcb.26.1.125
Abstract
Chromosomes and nuclei isolated from neutral formalin-fixed Vicia faba lateral roots were treated with trypsin, pepsin, RNase, or DNase. Only trypsin affected the morphology of the chromosomes and nuclei. The appearance of the chromosomes after trypsin digestion indicated that each chromatid contained four strands that could be seen with an ordinary light microscope. The experiments are interpreted as indicating that mitotic chromosomes of Vicia faba are multistranded and that the linear continuity of the chromosome is dependent on protein.Keywords
This publication has 29 references indexed in Scilit:
- Partial Synchronization of Nuclear Divisions in Root Meristems with 5-AminouracilScience, 1963
- CHROMOSOME DUPLICATION AND STRUCTURE AS DETERMINED BY AUTORADIOGRAPHYProceedings of the National Academy of Sciences, 1963
- Human chromosome uncoiling and dissociationAnnals of Human Genetics, 1962
- THE ANNUAL INVITATION LECTURE ULTRASTRUCTURE AND MOLECULAR ORGANIZATION OF GENETIC SYSTEMSCanadian Journal of Genetics and Cytology, 1961
- The Replication of DNABiophysical Journal, 1961
- Deoxyribonucleic Acid Side-Chain Model of the ChromosomesNature, 1958
- II. The Effect of Ionizing Radiation on ChromosomesThe Quarterly Review of Biology, 1957
- X-ray breakage experiments with endosperm I. Sub-chromatid breakageChromosoma, 1953
- A Selective Staining Method for the Basic Proteins of Cell NucleiProceedings of the National Academy of Sciences, 1953
- X-Ray and Ultraviolet Studies on Pollen Tube ChromosomeProceedings of the National Academy of Sciences, 1947