RED-CELL PROTEINS .1. 2-DIMENSIONAL MAPPING OF HUMAN-ERYTHROCYTE LYSATE PROTEINS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 53  (6) , 1121-1132
Abstract
Human erythrocyte lysate proteins were resolved into over 250 discrete spots by 2-dimensional electrophoresis using isoelectric focusing in the 1st dimension and electrophoresis in the presence of sodium dodecyl sulfate (SDS) in the 2nd. The overwhelming excess of Hb made such analyses difficult in the past. With the ISO-DALT 2-dimensional electrophoresis system, large numbers of red cell proteins can be mapped in the presence of Hb. When Hb and several other major proteins were removed by adsorption to DEAE-cellulose, additional minor components were seen, giving a total of over 275. With the use of purified preparations, the map positions of 5 red cell enzymes or their subunits were determined: pyruvate kinase, catalase, glucose-6-phosphate dehydrogenase, hypoxanthine phosphoribosyltransferase and carbonic anhydrase. These mapping techniques complement and extent those traditionally used to find human red cell protein variants.