The production of 15N‐labelled S‐adenosylmethionine and adenine by yeast biosynthesis
- 1 July 1968
- journal article
- review article
- Published by Wiley in Journal of Labelled Compounds and Radiopharmaceuticals
- Vol. 4 (3) , 230-239
- https://doi.org/10.1002/jlcr.2590040305
Abstract
A method for the biosynthesis of 15 N‐labelled S‐adenosylmethionine from 15 NH4+ and L‐methionine by yeast is reported. Culture conditions suitable for economy in the utilization of the isotope were developed, and the use of γ‐irradiated yeast (1 Mrad) for this purpose was explored. The irradiated yeast proved as effective as non‐irradiated yeast, but it did not offer any advantage. 15 N‐Adenine was obtained from S‐adenosylmethionine by hydrolysis; its isolation by ion exchange chromatography is described.Keywords
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