RAPID IMMUNOMAGNETIC PHENOTYPING OF CELLS
- 2 April 1989
- journal article
- research article
- Published by Wiley in International Journal of Immunogenetics
- Vol. 16 (2) , 169-175
- https://doi.org/10.1111/j.1744-313x.1989.tb00459.x
Abstract
SUMMARY: A new method for immunophenotyping of cells in suspension has been developed. The method is based on identification of cells that have formed rosettes with superparamagnetic particles (Dynabeads) conjugated with monoclonal antibodies directed against cell‐surface antigens. The method is extremely simple. Twenty‐five μl of cells are mixed with 5 μl of Dynabeads in U‐bottom microtitre wells. Following a I‐min centrifugation step, rosette formation can be inspected in the microscope. Staining of the cells before rosetting with acridine orange/ethidium bromide allows direct quantification of viable cells carrying a given marker. The method is limited to detection of cell‐surface antigens, and the results obtained are comparable to those seen with indirect immunofluorescence. The method may be used for phenotyping of leukaemia and other cancer cells, and can also be used for phenotyping of cells that can only be obtained in small numbers, such as spinal fluid cells.This publication has 10 references indexed in Scilit:
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