REMOVAL OF HEPATITIS-B SURFACE-ANTIGEN (HBSAG) FROM PLASMA FRACTIONS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 88  (1) , 91-101
Abstract
Endogenous or deliberately added HBsAg was removed and concentrated for assay from albumin and coagulation factor II, VII, IX and X concentrates as model plasma fractions. The concentrates carry considerable risk of causing hepatitis in transfused patients. The amount of antigen remaining in the fraction was estimated at < 1/10,000 of that detectable by the Ausria II radioimmunoassay and 1/100 of that found infectious when highly contaminated human sera were diluted and injected in chimpanzees. Batch fractionation methods with polyethylene glycol were used. The albumin yield was 96%, and the coagulation factors yield about 90%.

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