A Mouse Model for Cytomegalovirus Infection
Open Access
- 1 June 2001
- journal article
- unit
- Published by Wiley in Current Protocols in Immunology
- Vol. 43 (1) , 19.7.1-19.7.13
- https://doi.org/10.1002/0471142735.im1907s43
Abstract
This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (mCMV). Methods are included for propagating mCMV in cell cultures and for preparing a more virulent form of mCMV from salivary glands of infected mice. A plaque‐forming cell (PFC) assay is provided for measuring mCMV titers of infected tissues or virus stocks. In addition, a method is described for preparing the murine embryonic fibroblasts used for propagating mCMV and for the PFC assay.Keywords
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