Nitrate Reductase from Chlorella vulgaris

Abstract
Millimolar concentrations of tervalent Mn pyrophosphate can partially activate nitrate reductase which has been inactivated with NADH and HCN. The tervalent Mn complex is nevertheless not reduced by NADH in the presence of the enzyme, that is, it is not a substrate for the diaphorase moiety of the nitrate reductase. Ferric o-phenanthroline is a good diaphorase substrate, but fails to activate the inactive enzyme.

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