Induced loss in cerebral tissues of respiratory response to electrical impulses, and its partial restoration by additional substrates

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Abstract
The ability of guinea pig cerebral cortex slices to increase respiration with glucose as substrate, on applying electrical impulses, survived several adverse conditions. These included keeping the tissue for some hours at 0[degree], the prolonged passage of impulses with substrate, or metabolism in absence of substrate. Adequate application of impulses in absence of substrate changed the tissue so that its respiration no longer increased when glucose was provided and impulses were applied. Tissue so treated largely retained its ability to form more lactic acid from glucose in response to impulses. Respiratory response could be partly restored to the changed tissue by addition of fumaric or malic acids to normally adequate metabolic mixtures. Many other additions were ineffective. Inorganic phosphate and creatine phosphate were detd. in the initial and changed tissue, in the presence of various substrates, and have aided interpretation of the change induced by impulses in absence of substrate.