Fluorogenic and chromogenic substrates used in bacterial diagnostics.
- 1 September 1991
- journal article
- review article
- Vol. 55 (3) , 335-48
Abstract
Methods based on the application of chromogenic and fluorogenic substrates enable specific and rapid detection of a variety of bacterial enzymatic activities. By using these techniques, enzymatic reactions can be examined simultaneously or individually, either directly on the isolation plate or in cell suspensions. For this purpose, various testing principles and test kits for clinical and food microbiology have been introduced successfully during the last few years. In this paper we present a survey of different enzymes of microbial origin that are utilized for microbiological identification and differentiation and the corresponding methods. Particular emphasis is given to the examination of Escherichia coli and the description of the different techniques as used in routine analysis.This publication has 99 references indexed in Scilit:
- Glycosidase Activities of MycoplasmasZentralblatt für Bakteriologie, 1990
- Validation of flow cytometry for rapid enumeration of bacterial concentrations in pure culturesJournal of Applied Bacteriology, 1990
- Few-Minutes Tests for the Identification of Group A Streptococci and Enterococci with Chromogenic SubstratesZentralblatt für Bakteriologie, 1989
- Membrane filtration differentiation ofE. colifrom coliforms in the examination of waterJournal of Applied Bacteriology, 1989
- A New Chromogenic Assay for Direct Detection of StaphylocoagulaseZentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology, 1988
- Further development of simple tests to differentiate the legionellasJournal of Applied Bacteriology, 1988
- Rapid selective enumeration of bacteria in foods using a microcolony epifluorescence microscopy techniqueJournal of Applied Bacteriology, 1988
- Evaluation of a commercial β‐glucuronidase test for the rapid and economical identification ofEscherichia coliJournal of Applied Bacteriology, 1986
- Use of the direct epifluorescent filter technique for the enumeration of yeastsJournal of Applied Bacteriology, 1986
- The direct epifluorescent filter technique (DEFT): increased selectivity, sensitivity and rapidityJournal of Applied Bacteriology, 1985